The number of cancer stem cells in the tumor tissue and perifocal tissue of non-muscle invasive bladder cancer

Purpose of the study. Determine content cancer stem cells (CSCs) in tumor tissue (TT) and perifocal tissues (PT) muscle-non-invasive bladder cancer. Materials methods. We’ve examined fragments TT PT 7 (NMIBC) after surgical intervention – transurethral resection urinary (TUR). In samples that were used to obtain cell suspension using BD Medimachine apparatus (BD, USA) was treated with monoclonal antibodies CD45-APCCy7, CD44-FITC, CD133-??, CD24-PE assessed on flow cytometer FacsCantoII USA). The percentage CSC phenotypic markers determined analysis sample: CD45-CD44+CD24+, CD45-CD44+, CD45-CD24+, CD45-CD133+, CD45-CD44+CD133+. presence significant differences groups evaluated STATISTICA 13 software package between considered at p < 0.05. corresponding phenotype calculated relative total number cells. Results. numbers markers, such as CD24, CD44, 77 % 58 higher than PT: 18.3 ± 3.5 vs. 4.3 2.1, ? 0.044 15.5 5.3 6.5 0.8, 0.043, respectively. CD133+ 83 compared 41.6 12.1 22.7 7.6, 0.047. Conclusion. study CSCs is a promising direction for oncogenesis can be assess nature further development relapse / or progression disease, well various therapeutic approaches are aimed eliminating blocking pathways leading emergence maintenance this population patients NMIBC.